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How cells' sensing hairs are made

Body cells detect signals that control their behavior through tiny hairs on the cell surface called cilia. Serious diseases and disorders can result when these cilia do not work properly. New research from 嘿嘿视频 published this week in the journal Nature Cell Biology provides new insights into how these cilia are assembled.

鈥淚t鈥檚 a basic discovery, but with implications for understanding disease,鈥 said Jonathan Scholey, professor of molecular and cellular biology at 嘿嘿视频 and senior author of the study. Understanding how cilia are assembled and function can help scientists understand how conditions such as polycystic kidney disease and some growth and development disorders arise.

Cilia are built from bundles of microtubules made of a protein called tubulin. Scholey鈥檚 team discovered how two subunits of tubulin are winched into place by a type of protein motor belonging to a family of proteins called kinesins.

Scholey鈥檚 laboratory works with the soil roundworm Caenorhabditis elegans, whose cilia are essentially the same as those of humans and other mammals. Postdoctoral scholar Limin Hao, Scholey and their colleagues screened a collection of worms for those with mutations that affected the cilia.

They found two genes which, when mutated, caused worms to lose the tips of their cilia. Both genes turned out to be subunits of tubulin that are assembled into different parts of the microtubule: one is found all along the microtubule, and the other is concentrated at the tip.

The 嘿嘿视频 team used a combination of microscopy, molecular biology and computer modeling to study these two proteins. They found that both are moved into position by so-called kinesin-2 motors.

At one time, researchers had seen cilia as purely for movement, either moving a swimming cell through a fluid or moving fluid and suspended particles over the cell鈥檚 surface, Scholey said.

But in the late 1990s, researchers discovered that cilia were also involved in detecting signaling molecules that control gene expression and cell behavior. This signaling is vital for coordinating cell growth and the orderly development of tissues, for example in establishing left/right asymmetry in developing embryos.

鈥淩ecent work shows that cilia are ubiquitous in signaling,鈥 Scholey said. In earlier work, Scholey鈥檚 lab linked a defect in the kinesins that assemble cilia to Bardet-Biedl disease, which causes blindness, kidney disease and learning difficulties.

Coauthors of the paper are: Melanie Thein, Ingrid Brust-Mascher, Gul Civelekoglu-Scholey and Seyda Acar, all at 嘿嘿视频; Yun Lu and Shai Shaham, Rockefeller University, New York; and Bram Prevo, Vrije Universiteit Amsterdam, The Netherlands, who was a visiting scholar in Scholey鈥檚 laboratory. Hao is now a researcher at Harvard Medical School and Thein is now a writer/editor at the 嘿嘿视频 Cancer Center. The work was funded by grants from the National Institutes of Health.

Media Resources

Andy Fell, Research news (emphasis: biological and physical sciences, and engineering), 530-752-4533, ahfell@ucdavis.edu

Jonathan Scholey, Molecular and Cellular Biology, (530) 752-2271, jmscholey@ucdavis.edu

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